The following publications cover a variety of topics pertaining to molluscan shellfish aquaculture, fish and shellfish cryogenics, and fish and shellfish genetics.  Publication media range from extension fact sheets from the University of Florida (UF), USDA Southern Regional Aquaculture Center (SRAC) (of which UF is a part), World Aquaculture Society,

All University of Florida extension publications–covering a wide variety of topics including profitable and sustainable agriculture, environment and natural resources, 4-H and other youth programs, Florida-friendly landscapes, communities that are vibrant and prosperous, and economic well-being and life quality for individuals and families–can be found at the University’s Electronic Data Information Source (EDIS).


Refereed Publications 

A Simon, N. and H. Yang (2018). Cryopreservation of trochophore larvae from the hard clam Mercenaria mercenaria: Evaluation of the cryoprotectant toxicity, cooling rate and thawing temperature. Aquaculture Research, DOI: 10.1111/are.13752

L. Matthews, J. Murphy, C. Carmichael, H. Yang, T. R. Tiersch, M. Westerfield & Z. M. Varga. 2018. Changes to extender, cryoprotective medium, and In Vitro fertilization improve zebrafish sperm cell cryopreservation. Zebrafish, 15(3): 279-290

H. Yang, E. Hu, J. T. Buchanan & T. R. Tiersch. 2018. A Strategy for Sperm Cryopreservation of Atlantic Salmon, Salmo salar, for Remote Commercial-scale High-throughput Processing. Journal of the World Aquaculture Society. 49, 96-112.

H. Yang & X. Guo. 2018. Triploid hard clams Mercenaria mercenaria produced by inhibiting polar body I or polar body II. Aquaculture Research, 49 (1): 449-461.

H. Yang. 2017. Application of germplasm preservation in breeding programs for molluscan shellfish aquaculture and restoration. Bulletin of Japan Fisheries Research and Education Agency. 45, 15-20.

L. Torres, Y. Liu, A. Guitreau, H. Yang & T. R. Tiersch. 2017. Challenges in development of sperm repositories for biomedical fishes: Quality control in small-bodied species. Zebrafish. 14, 552-560.

S. Laramore, L. Sturmer, R. Baptiste, H. Yang, C. Sinacore & E. Urban-Gedamke.. 2017. Fatty acid composition of adult and larval sunray venus clams Macrocallista nimbosa: Environmental and gametogenic impacts. Journal of Shellfish Research. 36, 403-416.

L. He, X. Zhang, Y. Huang, H. Yang, Y. Wang & Z. Zhang. 2017. The characterization of RHEB gene and its responses to hypoxia and thermal stresses in the small abalone Haliotis diversicolor. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 210, 48-54.

H. Yang, J. Daly, and T. R. Tiersch. 2016. Determination of sperm concentration using flow cytometry with simultaneous analysis of sperm plasma membrane integrity in zebrafish Danio rerio.  Cytometry Part A.  89a: 350-356.

E Hu, R. Cuevas-Uribe, H. Yang, R. Sanderson, A. O. Gill, H. S. Daniel, and T. R. Tiersch. 2016. High-throughput cryopreservation of sperm from sex-reversed southern flounder, Paralichthys lethostigma.  Journal of World Aquaculture Society. 47(4): 555-565.

H. Yang, J. Daly, C. Carmichael, J. Matthews, Z. M. Varga, and T. R. Tiersch. 2016. A procedure-spanning analysis of plasma membrane integrity for assessment of cell viability in sperm cryopreservation of zebrafish Danio rerio. Zebrafish. 13(2): 144-151.

H. Yang, Y. Wang, X. Guo and T. R. Tiersch. 2015. Production of inbred larvae through self-fertilization using oocytes and cryopreserved sperm from the same individuals after sex reversal in eastern oyster Crassostrea virginica.  Aquaculture Research.  46: 2153-65.

H. Yang, X. Guo, J. Supan, and T. R. Tiersch.  2013. Non-lethal sperm collection and cryopreservation in the eastern oyster Crassostrea virginica.  Journal of Shellfish Research.  32: 429-437.

H. Yang, G. M. Savage, L. Hazlewood, R. B. Walter, and T. R. Tiersch.  2012. Offspring production with cryopreserved sperm from a live-bearing fish Xiphophorus maculatus and implications with females.  Comp Biochem Physiol C Toxicol Pharmacol.  155: 55-63

T. R. Tiersch, and H. Yang.  2012. Environmental salinity-induced shifts in sperm motility activation in Fundulus grandis.  Aquaculture.  324-325: 145-150.

H. Yang, R. Cuevas-Uribe, M. G. Savage, R. B. Walter, and T. R. Tiersch.  2012. Sperm cryopreservation in live-bearing Xiphophorus fishes: offspring production from Xiphophorus variatus and strategies for establishment of sperm repositories.  Zebrafish.  9: 126-134.

H. Yang, E Hu, R. Cuevas-Uribe, J. Supan, X. Guo, and T. R. Tiersch.  2012. High-throughput sperm cryopreservation of eastern oyster Crassostrea virginica.  Aquaculture.  344: 223-230

E Hu, H. Yang, and T. R. Tiersch.  2011. High-throughput cryopreservation of spermatozoa of blue catfish Ictalurus furcatus: Establishment of an approach for commercial-scale processing.  Cryobiology.  1: 74-82

R. Cuevas-Uribe, Jonathan Daly, H. Yang, R. B. Walter, M. G. Savage, and T. R. Tiersch.  2011. Production of F1 offspring with vitrified sperm from a live-bearing fish, the green swordtail Xiphophorus hellerii.  Zebrafish.  8: 167-179.

T. R. Tiersch, H. Yang, and E Hu.  2011. Outlook for development of high-throughput cryopreservation for small-bodied biomedical model fishes. Comp Biochem Physiol C Toxicol Pharmacol.  154: 76-81.

H. Yang, M. Norris, R. Winn, and T. R. Tiersch.  2010. Evaluation of cryoprotectant and cooling rate for sperm cryopreservation in the euryhaline fish medaka Oryzias latipes.  Cryobiology.  61: 211-219.

E Tan, H. Yang, and T. R. Tiersch.  2010. Determination of sperm concentration for small-bodied biomedical model fishes by use of microspectrophotometry.  Zebrafish.  7: 233-240.

H. Yang, and T. R. Tiersch.  2009. Current status of sperm cryopreservation in biomedical research fish models: zebrafish, medaka, and Xiphophorus.  Comp Biochem Physiol C Toxicol Pharmacol.  149 (2): 224-232.

H. Yang, and T. R. Tiersch.  2009. Sperm motility initiation and duration in a euryhaline fish, medaka Oryzias latipes.  Theriogenology.  72: 386-392.

H. Yang, L. Hazlewood, R. B. Walter, and T. R. Tiersch.  2009. Sperm cryopreservation of a live-bearing fish, Xiphophorus couchianus: Male-to-male variation in post-thaw motility and production of F(1) hybrid offspring.  Comp Biochem Physiol C Toxicol Pharmacol.  149: 233-239.

H. Yang, L. Hazlewood, S. J. Heater, P. A. Guerre, R. B. Walter, and T. R. Tiersch.  2007. Production of F1 interspecies hybrid offspring with cryopreserved sperm from a live-bearing fish, the swordtail Xiphophorus helleri.  Biology of Reproduction.  76: 401-406. Honored cover page of this issue.

H. Yang, C. Carmichael, Z. M. Varga, and T. R. Tiersch.  2007. Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio.  Theriogenology.  68: 128-136.

H. Yang, L. Hazlewood, R. B. Walter, and T. R. Tiersch.  2006. Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail Xiphophorus helleri.  Cryobiology.  52: 209-218.

H. Yang, and X. Guo.  2006. Tetraploid induction by inhibiting mitosis I with heat shock, cold shock, and nocodazole in the hard clam Mercenaria mercenaria (Linnaeus, 1758).  Marine Biotechnology.  8: 501-510.

H. Yang, and X. Guo.  2006. Polyploid induction by heat shock-induced meiosis and mitosis inhibition in the dwarf surfclam, Mulinia lateralis Say.  Aquaculture.  252: 171-182.

H. Yang, and X. Guo.  2004. Tetraploid induction by meiosis inhibition in the dwarf surfclam Mulinia lateralis: Effects of cytochalasin B duration.  Aquaculture Research.  35: 1187-1194.

N. Gong, H. Yang, G. Zhang, B. J. Landau, and X. Guo.  2004. Chromosome inheritance in triploid Pacific oyster Crassostrea gigas. Heredity.  93: 408-415.

H. Yang, Li Li, and X. Guo.  2001. Preliminary study on inducing polyploid in Japanese scallop Patinopecten yessoensis by inhibiting polar bodies with cytochalasin B.  Acta Zoologica Sinica.  47: 459-464.

H. Yang, X. Guo, and F. Zhang.  2000. Triploid and tetraploid Zhikong scallop, Chlamys farreri Jones et Preston, produced by inhibiting polar body I.  Marine Biotechnology.  2: 466-475.

H. Yang, H. Que, Y. He, and F. Zhang.  2000. Chromosome segregation in fertilized eggs from zhikong scallop Chlamys farreri (Jones & Preston) following polar body 1 inhibition with cytochalasin B.  Journal of Shellfish Research.  19: 101-105.

H. Yang, T. Gallivan, X. Guo, and S. K. Allen.  2000. A method for preserving oyster tissue samples for flow cytometry.  Journal of Shellfish Research.  19: 835-839.

H. Yang, X. Guo, Z. Chen, and Y. Wang.  1999. Tetraploid induction by inhibiting mitosis I in Zhikong scallop Chlamys farreri.  Chinese Journal of Oceanology and Liminology. 17: 350-358.

H. Yang, S. Tong, and Z. Wang.  1998. Review about the digestive enzymes from fisheries animals.  Journal of Dalian Fisheries University.  13: 64-71.

H. Yang, S. Tong, and Z. Wang.  1998. Properties of amylase and algalase of Pacific abalone Haliotis didcus hannai Ino.  Journal of Fisheries of China.  22: 345-351.

H. Yang, R. Wang, X. Guo, and Z. Yu. 1997. Tetraploid induction of Zhikong scallop Chlamys farreri I: Cytochalsin B. Journal of Ocean University of Qingdao. 27: 166-172.

H. Yang, S. Tong, and Z. Wang.  1997. Study of the protease of abalone Haliotis discus hannai Ino.  Journal of Fisheries of China.  21(2): 128-133.

Extension style publications

H. Yang, L.N. Sturmer, and S. Baker.  2015. Molluscan Shellfish Aquaculture and Production.  UF EDIS.

X. Guo, H. Yang, and J. Kraeuter.  2001. Triploid and tetraploid technology for hard clam aquaculture.  The Jersey Shoreline.  20: 6-9.

Book Chapter

H. Yang, R. B. Walter, and T. R. Tiersch.  2012. Current status of sperm cryopreservation in the genus Xiphophorus. Pp 403-414. In: Viviparous Fishes II. New Life Publications.  Homestead, Florida.

H. Yang, and Terrence R. Tiersch.  2011. Sperm Cryopreservation in Biomedical Research Fish Models. Pp 439-454. In: Cryopreservation in Aquatic Species.  World Aquaculture Society.  Baton Rouge, LA.

H. Yang, and Terrence R. Tiersch.  2011. Application of Computer-assisted Sperm Analysis (CASA) to Aquatic Species. Pp 240-254. In: Cryopreservation in Aquatic Species. World Aquaculture Society. Baton Rouge, LA.

X. Guo, Y. Wang, Z. Xu, and H. Yang.  2009. Chromosome set manipulation in shellfish. Pp 165-194. In: New Technologies in Aquaculture: Improving Production Efficiency, Quality and Environmental Management. : Woodhead Publishing Limited. pp. 165-194. Woodhead Publishing Limited.  Abington, Cambridge.

T. R. Tiersch, H. Yang, J. A. Jenkins, and Q. Dong.  2007. Sperm cryopreservation in fish and shellfish. Pp 493-508. In: Spermatology (Soc Reprod Fertil Supplement 65). Nottingham University Press.  Nottingham.

David Randall, and H. Yang.  2004. The role of hypoxia, b-naphthoflavone and the aryl hydrocarbon receptor nuclear translocator in the inhibition of reproduction in fish. Pp 253-261. In: Proceedings of the 7th International Symposium on Fish Physiology, Toxicology, and Water Quality. Athens.  Environmental Protection Agency, Ecosystems Research Division. Athens, GA.

X. Guo, H. Yang, H. Que, Zhe Xu, and Z. Wang. 1999. The Cytogenetics of Mollusks. Pp 101-125. In: The Advances of Marine Biotechnology. Oceanology Press.  Beijing.